ABSTRACT
The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length, intracellular cAMP content, and Gap-43 expression in N1E-115 cells in vitro by use of microscopy, LANCE(tm) cAMP 384 kit, and Western blot analysis, respectively. Our results showed that quercetin could increase the neurite length in a concentration-dependent manner, but had no effect on the numbers of cells. Quercetin significantly increased the expression of cellular cAMP in a time- and concentration-dependent manner. The Gap-43 expression was up-regulated in a time-dependent manner. In conclusion, quercetin could promote neurite growth through increasing the intracellular cAMP level and Gap-43 expression.
Subject(s)
Cell Line , Cyclic AMP , Metabolism , GAP-43 Protein , Metabolism , Nerve Regeneration , Neurites , Plant Extracts , Pharmacology , Quercetin , Pharmacology , Signal TransductionABSTRACT
A new benzene derivative microintegerrin C (1) and a new norsesquiterpenoid microintegerrin D (2), along with six known compounds (3-8), were isolated and identified from stems and leaves of Micromelum integerrimum by various chromatographies such as silica gel, Sephadex LH-20, RP-18 column chromatography and HPLC. Their structures were mainly identified based on the spectral data analysis such as 1D-, 2D-NMR and HR-EI-MS. All known compounds were isolated from this plant for the first time.
Subject(s)
Chromatography, High Pressure Liquid , Plant Leaves , Chemistry , Plant Stems , Chemistry , Rutaceae , Chemistry , SesquiterpenesABSTRACT
AIM@#To investigate the chemical and bioactive constituents from the stems and leaves of Micromelum integerrimum.@*METHOD@#The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and HPLC. Their structures were mainly elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy and mass spectrometry. Their cytotoxicity and antimicrobial activities were tested by the SRB and turbidimetric methods, respectively.@*RESULTS@#Two new phenylpropanoids and two known coumarins were obtained, and their structures were identified as microintegerrin A (1), microintegerrin B (2), scopoletin (3), and scopolin (4). All of the compounds were tested for their cytotoxicity against three cancer cell lines (HeLa, A549, and BGC-823) and for antimicrobial activity against the fungus Candida albicans and the bacterium Staphylococcus aureus.@*CONCLUSION@#Two new phenylpropanoids 1 and 2 were isolated and identified from the stems and leaves of M. intgerrimum. None of the compounds showed cytotoxic or antimicrobial activity at the tested concentration of 20 μg·mL(-1).
Subject(s)
Humans , Candida albicans , Coumarins , Pharmacology , Glucosides , Pharmacology , HeLa Cells , Molecular Structure , Phenylpropionates , Chemistry , Pharmacology , Plant Extracts , Chemistry , Pharmacology , Plant Leaves , Plant Stems , Rutaceae , Chemistry , Scopoletin , Pharmacology , Staphylococcus aureusABSTRACT
Eleven compounds were isolated and purified from the barks extract of Nothopanax delavayi and their structures were identified as serratagenic acid-3-O-alpha-L-arabinopyranosyl-28-O-beta-D-glucopyranosyl ester (1), serratagenic acid-3-0-alpha-L-arabi-nopyranosyl-28-O-[alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester (2), serratagenic acid (3), serratagenic acid-3-O-alpha-L-arabinopyranoside (4), serratagenic acid-beta-O-beta-(2', 4'-O-diacetyl) -D-xylopyranosyl-28-O-[alpha-L-rhamnopy-ranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->46)-beta-D-glucopyranosyl] ester (5), serratagenic acid-3-O-alpha-(4'-O-acetyl)-L-arabino pyrano-syl-28-0- [-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester(6), serratagenic acid-3-O-alpha-(2'-O-acetyl)-L-arabinopyranosyl-28-O-[-alpha-L-rhamnopyranosyl- (1-->4) -beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester(7), serratagenic acid-3-0-beta-D-xylopyranosyl-28-O-[-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester (8), protocatechuic acid (9), ethyl caffeate (10) and caffeic anhydride (11) by physicochemical properties and spectroscopic data analysis. Among them, compounds 3-4 and 9-11 were firstly isolated from the genus Nothopanax, and compounds 5-8 were isolated from this plant for the first time.
Subject(s)
Araliaceae , Chemistry , Drugs, Chinese Herbal , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Bark , ChemistryABSTRACT
AIM@#To study the chemical constituents from the aerial parts of Trachelospermum jasminoides.@*METHODS@#Compounds were isolated by various chromatographic methods and their structures were elucidated on the basis of spectral techniques. In addition, the inhibitory activity against HepG2 and HL-60 of compound 1 was evaluated.@*RESULTS@#Five compounds were isolated and identified as 3', 7-dimethoxyisoflavanone-4', 5-di-O-β-D-glucopyranoside (1), tracheloside (2), nortrachelogenin 5'-C-β-glucopyranoside (3), luteolin-4'-O-β-D-rutinoside (4), and luteolin-7-O-β-D-glucopyranoside (5).@*CONCLUSION@#Compound 1 is a new isoflavonoid glycoside. It showed weak inhibitory activity against HepG2 and HL-60 cancer cells with IC50 values of 131.5 and 58.2 μmol·L(-1), respectively.
Subject(s)
Humans , Apocynaceae , Chemistry , Cell Line, Tumor , Cell Proliferation , Flavonoids , Chemistry , Pharmacology , Glycosides , Chemistry , Pharmacology , Molecular Structure , Plant Components, Aerial , Chemistry , Plant Extracts , Chemistry , PharmacologyABSTRACT
Fourteen compounds were isolated and purified from the ethyl acetate of the ethanol extract of Shiaria bambusicola by various chromatographic methods, and their structures were elucidated by spectral techniques and physicochemical properties as hypocrellin A (1), hypocrellin B (2), hypocrellin C (3), hypomycin A (4), ergosterol (5), ergosterol peroxide (6), (22E, 24R)-5alpha, 8alpha-epidioxy-6,9(11),22-trien-3beta-ol (7), ergosta-7, 24(28)-dien-3beta-ol (8), (22E, 24R)-ergost-7, 22-dien-3beta, 5alpha, 6beta-triol (9), (22E,24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol-3-O-palmitate (10), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol-6-O-palmitate (11), 1-O-monostearin (12), 1, 3-O-distearin (13), and mannitol (14). Among them, compounds 7-13 were firstly isolated from this genus.
Subject(s)
Acetates , Chemistry , Ascomycota , Chemistry , Biological Factors , Chemistry , Molecular Structure , Spectrometry, Mass, Electrospray IonizationABSTRACT
Ten Amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Their structures were identified as oxovittatine (1), apohaemanthamine (2), 9-O-demethylhomolycorine N-oxide (3), incartine (4), ismine (5), 6-O-methylpretazettine (6), tazettine (7), ungeremine (8), homolycorine (9), and O-methyllycorenine (10) by spectroscopic data analyses. Compound 1 was a new natural product. Compounds 2 and 3 were reported form the genus Lycoris for the first time and compounds 4-6 were isolated form the title plant for the first time.
Subject(s)
Alkaloids , Chemistry , Lycoris , Chemistry , Plant Extracts , Chemistry , Plant Roots , ChemistryABSTRACT
To investigate the chemical constituents of the leaves of Adinandra nitida, several column chromatography methods were used to isolate the chemical constituents of this plant. The structures were elucidated on the basis of spectral data. Six compounds were isolated and identified as 2alpha, 3alpha, 19alpha-trihydroxy-olean-12-en-28-oic acid-28-O-beta-D-glucopyranoside (1), arjunetin (2), sericoside (3), glucosyl tormentate (4), nigaichigoside F1 (5) and arjunglucoside I (6), separately. Compound 1 is a new compound, and compounds 2 -6 were isolated from A. nitida for the first time.
Subject(s)
Glucosides , Chemistry , Molecular Structure , Oleanolic Acid , Chemistry , Plant Leaves , Chemistry , Plants, Medicinal , Chemistry , Saponins , Chemistry , Theaceae , ChemistryABSTRACT
Sesame (Sesamum indicum L. ) belongs to Pedaliaceae, and its dry flowers have been used to cure alopecia, frostbite and constipation as a Traditional Chinese Medicine. Interestingly, the Flos Sesamum indicum L. was usually used to cure verruca vulgaris and verruca plana in folk of China, and showed a pleasant result. Previous chemical investigations of this plant mainly concentrate on its seeds, showed the presence of proteins and fat oils, herein we make a systematic chemical research on the dry flowers of this plant. Column chromatography including silica gel, C18 and Sephadex LH-20 were used to separate the chemical constituents and the structures were determined by chemical and spectroscopic methods. Ten compounds were isolated from the 95% ethanol extract of the plant and elucidated as latifonin (1), momor-cerebroside (2), soya-cerebroside II (3), 1-O-beta-D-glucopyranosyl-(2S, 3S, 4R, 5E,9Z)-2-N-(2'-hydroxytetracosanoyl) 1,3,4-trihydroxy-5,9-octadienine (4), 1-O-beta-D-glucopyranosyl-(2S, 3S, 4R, 8Z)-2-N-(2' R) 2'-hydroxytetracosanoyl) 3,4-dihydroxy-8-octadene (5), (2S, 1" S) -aurantiamide acetate (6), benzyl alcohol-O-(2'-O-beta-D-xylopyranosyl, 3'-O-beta-D-glucopyranoside)-beta-D-glucopyranoside (7), beta-sitosterol (8), daucosterol (9) and D-galacititol (10). Among them, 4 is a new compound, and others were isolated from the flowers of the plant for the first time. Compounds 2 to 4 belong to cerebroside, which is rare to be found in land plants and was proved to possess many bioactivities.
Subject(s)
Cerebrosides , Chemistry , Flowers , Chemistry , Glycolipids , Chemistry , Molecular Conformation , Molecular Structure , Plants, Medicinal , Chemistry , Sesamum , Chemistry , Sitosterols , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents of the root barks of Periploca sepium.</p><p><b>METHOD</b>Column chromatographic techniques were used to isolate the chemical constituents. NMR and MS methods were employed for their structural elucidation.</p><p><b>RESULT</b>Eight compounds were isolated and identified as isovanillin (1), vanillin (2), 4-methoxysalicylic acid (3), (24R)-9, 19-cycloart-25-ene-3beta, 24-diol (4), (24S)-9, 19-cycloart-25-ene-3beta, 24-diol (5), cycloeucalenol (6), beta-amyrin acetate (7) and alpha-amyrin (8).</p><p><b>CONCLUSION</b>Compounds 1-6 were isolated from this plant for the first time.</p>
Subject(s)
Benzaldehydes , Chemistry , Molecular Structure , Periploca , Chemistry , Phytosterols , Chemistry , Plant Bark , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Pseudostellaria heterophylla.</p><p><b>METHOD</b>The compounds were isolated by silica gel and Sephadex LH-20 column chromatography and purified by recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectral analysis.</p><p><b>RESULT</b>Seven compounds were identified as 2-minalin (1) , 3-furfuryl pyrrole-2-carboxylate (2), ursolic acid (3), acacetin (4), luteolin (5), acacetin 7-O-beta-D-glucopyranosyl (6-->1)-alpha-L-rhamnopyranoside (6).</p><p><b>CONCLUSION</b>Among them, compounds 3-6 were isolated from the plant for the first time.</p>
Subject(s)
Caryophyllaceae , Chemistry , Chromatography, Gel , Flavones , Chemistry , Luteolin , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents from the branch of Broussonetia papyrifera.</p><p><b>METHOD</b>Column chromatographic methods were used to isolate the chemical constituents. ESI-MS and NMR methods were employed for their structural elucidation.</p><p><b>RESULT</b>Six compounds were isolated and identified as (2S)-7, 3'-dihydroxy-4'-methoxyflavan (1), ergosterol peroxide (2), D-galacitol (3), sulfuretin (4), liriodendrin (5), graveolone (6), respectively.</p><p><b>CONCLUSION</b>Compounds 1-6 were isolated from the plant for the first time.</p>
Subject(s)
Benzofurans , Chemistry , Broussonetia , Chemistry , Ergosterol , Chemistry , Flavonoids , Chemistry , Furans , Chemistry , Glucosides , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Lygodium japonicum.</p><p><b>METHOD</b>Column chromatographic techniques were used for isolation and purification of chemical constituents of this plant and their structures were identified by spectroscopic analysis.</p><p><b>RESULT</b>Eight compounds were isolated and identified as tilianin (I), kaempferol-7-O-alpha-L-rhamnopyranoside (II), kaempferol (III), p-coumaric acid (IV), hexadecanoic acid 2, 3-dihydroxy-propyl ester (V), daucosterol (VI), beta-sitosterol (VII, and 1-hentriacontanol (VIII) respectively.</p><p><b>CONCLUSION</b>Compounds I, II, V, VI, VII, VIII were isolated from L. japonicum for the first time, compounds I, II, V were isolated from genus Lygodium for the first time.</p>
Subject(s)
Ferns , Chemistry , Flavonoids , Chemistry , Glycosides , Chemistry , Kaempferols , Chemistry , Plant Components, Aerial , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Bombyx batryticatus.</p><p><b>METHOD</b>The chemical constituents were isolated from the title materials by column chromatography using silica gel, purified by crystallization, and identified by spectroscopic methods.</p><p><b>RESULT</b>Seven compounds were isolated and identified.</p><p><b>CONCLUSION</b>Six compounds were isolated from Bombyx batryticatus for the first time.</p>